FIGURE 4.

PG positively regulates ROP6-mediated endocytosis regulation. (A) FM4-64 uptake in the seedlings of Col-0, rop6-2 and rop6^CA. Seedlings of Col-0 and rop6-2 were treated with indicated amounts of PG, and then stained with FM4-64 to observe endocytosis process. The amount of PG that each treatment used was shown on the top. The upper lane shows the FM4-64 uptake in the seedlings of Col-0. The lower lane shows the FM4-64 uptake in the seedlings of rop6-2. rop6^CA was stained with FM4-64 and observed for endocytosis process. Scale bar represents 5 μm. (B) Ratio quantitation analysis of FM4-64 signal in (A) in bar graph. The FM4-64 signal in the cytoplasm and the plasma membrane was performed measurement separately using ImageJ software, and then the ratio was calculated (n > 20). (C) Ratio quantitation analysis of FM4-64 signal between Col-0 and rop6-2 in (B). The bar represents the mean and the error bar represents the standard error. The data were calculated from at least three independent experiments. The statistical significance was analyzed by a Student’s t-test and the significant differences (P ≤ 0.05) are indicated by lowercase letters.