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. 2018 Mar 15;9:202. doi: 10.3389/fphys.2018.00202

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Copyright © 2018 Cury-Boaventura, Gorjão, de Moura, Santos, Bortolon, Murata, Borges, Momesso, Dermargos, Pithon-Curi and Hatanaka.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CD25 (A), CD28 (B), and CTLA-4 (C) expression on lymphocytes before and immediately after the futsal match. The cells were pelleted and labeled with APC-conjugated anti-CD25 (1:50), FITC-conjugated anti-CD28, and FITC-conjugated anti-CTLA-4 antibodies. Cells were analyzed by flow cytometry. Negative control cells were incubated with a labeled non-reactive control antibody. The values are presented as the means ± standard error of 16 players. ^*p < 0.05 for the comparisons between before and after the match.