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. 2018 Mar 21;8:4971. doi: 10.1038/s41598-018-23366-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of glucose concentration over growth of GAS in THY and C media. M1T1 GAS 5448 was grown in either THY (A) or C media (B), with growth (black, OD₆₀₀) and total sugar (glucose) concentration (red, mg/dl measured every hour as described in Methods). Data represent the average of three replicates, shown with the standard error of the mean. The right Y axis (red) represent different levels of glucose concentrations in (A) and (B). Arrows indicate sampling point for RNA isolation and dashed lines show the glucose level at that time point.