Table 4.
Bacterial strains and plasmids used in this study.
| Strain/Plasmid | Relevant genotype | Reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | hsdR17 recA1 gyrA endA1 relA1 | 72 |
| C41[DE3] | F- ompT gal dcm hsdSB (rB−mB−)(DE3) | 57 |
| C43[DE3] | F- ompT gal dcm hsdSB (rB−mB−)(DE3) | 57 |
| S. pyogenes | ||
| 5448 | M1T1 | 54 |
| 5448.930 | ∆mga mutant in 5448 (insertional inactivation) | This study |
| 5448.930 R | mga + 5448, cured strain | This study |
| KM16.1 | ∆mga mutant in 5448 (transposon) | This study |
| GA40634 | M4 GAS, clinical isolate | 73 |
| KSM547 | ∆mga mutant in GA40634 | 38 |
| SF370 | M1 GAS | 74 |
| KSM165L | ∆mga mutant in SF370 | 27 |
| Plasmids | ||
| pCRS | Temperature-sensitive conditional vector; SpR | 23 |
| pJRS525 | Replicating vector for GAS with SpR | 36 |
| pKSM807 | WT mga1 under native Pmga1 | 34 |
| pKSM808 | WT mga4-his6 (GA40634) under native Pmga4 | 34 |
| pKSM809 | WT mga1-his6 under native Pmga1 | 34 |
| pKSM871 | H204A/H270A/H324A mga4-his6 under native Pmga4 | 3 |
| pKSM930 | pCRS construct; insertional inactivation of mga | This study |
| pLZ12-Spec | Broad host range cloning vector, SpR | 75 |
| pMga1-His | WT mga1 (SF370) with C-terminal His6 | 34 |
| pUC19 | ColE1 ori ApR lacZα | 76 |