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. 2018 Mar 15;11:57. doi: 10.3389/fnmol.2018.00057

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Copyright © 2018 Bruce, Gorkhali, Given, Coates, Boyle, Macklin and Eckel.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chemokine and cytokine production in LPL KD microglia. Ray biotech mouse neuro array was used to determine differences in chemokine expression in media from (A) BV-2 202 or (B) BV-2 553 cells. (C–F) Comparative gene expression between control (BV-2 202) and LPL KD (BV-2 553) cells (N = 4 per group). (G) Ex vivo brain slices following incubation with conditioned media (CM) from either control (WT CM) or LPL KD (KD CM) for 24 h, characterizing Plp-eGFP MAG, neurofilament or Iba1 expression (N = 3 per group). ***P < 0.001 vs. CONT.

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