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. 2018 Mar 21;8(3):35. doi: 10.1038/s41408-018-0062-y

Fig. 3. IGLL5 is translocated and DERL3 is overexpressed in multiple myeloma.

Fig. 3

a Schematic of validated t(14;22) translocation. (Left) WT chromosomes 14 and 22 with horizontal lines indicating location of breakpoints within the IGH and IGLL5 loci, respectively. (Right) Two derivative (der) chromosomes, each retaining a portion of its respective IGH or IGLL5 gene. Cancer-associated genes within 1 Mb of breakpoint on der(14) are shown. b PCR validation of t(14,22) translocation. Oligos specific to each breakpoint used in PCR reactions (top). Oligos specific to the small regions deleted on der(14) and der(22) were designed to detect non-translocated allele (bottom). T tumor, N germline (peripheral blood) control. cDERL3 expression across 84 MM patients. Red circle indicates sample in which putative (non-validated) t(14;22) translocation was detected. FPKM Fragments Per Kilobase of transcript per Million mapped reads