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. 2018 Mar 21;8:4955. doi: 10.1038/s41598-018-23029-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunoproteomic analyses of M. catarrhalis OMV preparations. After 2D-gel electrophoresis OMV proteins were visualized by Coomassie staining (A,E). 2D immunoblots probed with homologous antisera (B,F), stronger cross-reactive antisera (C,G) and weaker cross-reactive antisera (D,H). Molecular size markers are indicated on the left. The exposure times for chemiluminescent substrate were exactly the same for each presented blot. Gel and blot images are cropped and brightness/contrast-adjusted for better readability. Original images are shown in Supplementary Fig. S2.