. 2018 Mar 21;8:4955. doi: 10.1038/s41598-018-23029-7

Table 1.

Immunogenic proteins determined by LC-MS/MS of excised spots found by immunoblotting on 2DE gels.

Protein description and name	Functional category¹	MW (kDa)²	Strain	Accession number	Sequence coverage (%)	Score³
Outer membrane protein CD (OMP CD)	Cell envelope biogenesis, outer membrane¹, cell binding, serum resistance	48.3	Mc6	SQ01_01315	23–56	328–1213
Outer membrane protein CD (OMP CD)		48.3	Mc8	SQ02_01320	25–44	464–1064
Outer membrane protein E (OMP E)	Lipid metabolism¹, fatty acid transport, serum resistance	49.3	Mc6	SQ01_04940	69–71	1473–1720
Outer membrane protein E (OMP E)	Lipid metabolism¹, fatty acid transport, serum resistance	49.3	Mc8	SQ02_04980	45–67	1105–1995
Outer membrane protein (CopB), TonB-dependent siderophore receptor	Inorganic ion transport and metabolism¹, serum resistance	82.8	Mc6	SQ01_06490	30–58	1115–2204
	Inorganic ion transport and metabolism¹, serum resistance	82.8	Mc8	SQ02_06550	33–50	1260–2019
Ubiquitous surface protein A (UspA1)	Cell division and chromosome partitioning¹, cell binding, serum resistance	93.3	Mc6	SQ01_03435	24–41	596–1011
Ubiquitous surface protein A (UspA1)		93.3	Mc8	SQ02_03470	22–23	787–782
Outer membrane porin (OMP M35)	Cell envelope biogenesis, outer membrane¹, transport and binding proteins	38.0	Mc6	SQ01_03260	38	533
Outer membrane porin (OMP M35)		38.0	Mc8	SQ02_03275	78	1286
Transferrin binding protein B (TbpB)	transport and binding proteins	76.2	Mc6	SQ01_05710	22–38	884–1140
Transferrin binding protein B (TbpB)	transport and binding proteins	76.2	Mc8	SQ02_05755	21	710
Lactoferrin binding protein (LbpB)	Transport and binding proteins	100.1	Mc6	SQ01_07800	10	353
Lactoferrin binding protein (LbpB)	Transport and binding proteins	100.1	Mc8	SQ02_07885	13	558
Haemoglobin-utilization protein, TonB-dependent receptor, (MhuA)	Inorganic ion transport and metabolism¹, transport and binding proteins	107.6	Mc6	SQ01_05485	37–40	1422–1460
		107.6	Mc8	SQ02_05535	ND	ND
Structural protein (MipA)	Cell envelope biogenesis, outer membrane¹	28.6	Mc6	SQ01_04155	53–60	498–577
Structural protein (MipA)	Cell envelope biogenesis, outer membrane¹	28.6	Mc8	SQ02_04180	75	799
Fe(3+) ABC transporter substrate-binding protein (FbpA)	Inorganic ion transport and metabolism¹	35.7	Mc6	SQ01_07425	ND	ND
Fe(3+) ABC transporter substrate-binding protein (FbpA)	Inorganic ion transport and metabolism¹	35.7	Mc8	SQ02_07515	58–73	849–1080
Molecular chaperone (GroEL)	Posttranslational modification, protein turnover, chaperones¹	57.4	Mc6	SQ01_02125	ND	ND
Molecular chaperone (GroEL)		57.4	Mc8	SQ02_02135	60	1721
Dihydrolipoamide dehydrogenase (Lpd)	Energy production and conversion¹	51.1	Mc6	SQ01_00050	29	737
Dihydrolipoamide dehydrogenase (Lpd)	Energy production and conversion¹	51.1	Mc8	SQ02_00050	46	1204
Hypothetical protein with TPR domain (HP Mc4)	General function prediction only¹	30.1	Mc6	SQ01_03330	46	554
Hypothetical protein with TPR domain (HP Mc4)	General function prediction only¹	30.1	Mc8	SQ02_03345	62	758
Outer membrane beta-barrel protein OMPJ)	Cell envelope biogenesis, outer membrane¹	18.9	Mc6	SQ01_07075	ND	ND
Outer membrane beta-barrel protein OMPJ)	Cell envelope biogenesis, outer membrane¹	18.9	Mc8	SQ02_07135	34	246
Polyisoprenoid-binding periplasmic protein (YceI)	General function prediction only¹	21.2	Mc6	SQ01_05855	ND	ND
Polyisoprenoid-binding periplasmic protein (YceI)	General function prediction only¹	21.2	Mc8	SQ02_05915	63	528
Membrane protein (OMP G1b)	Cell envelope	26.9	Mc6	SQ01_02650	29	448
Membrane protein (OMP G1b)	Cell envelope	26.9	Mc8	SQ02_02660	ND	ND
Membrane protein, Opa-like protein A (OlpA)	Cell envelope biogenesis, outer membrane¹	23.3	Mc6	SQ01_01010	50	296
Membrane protein, Opa-like protein A (OlpA)	Cell envelope biogenesis, outer membrane¹	23.3	Mc8	SQ02_01010	ND	ND

¹Functional category according to search results of Cluster of Orthologous Groups (COGs); other descriptions were taken from various references;

²Theoretical molecular mass was determined by Mascot;

³Score ranges represent MS/MS ion scores determined by peptide mass fingerprinting. Only scores deemed to be significant by Mascot (p < 0.05) were presented.

ND - not done as there was no reactivity observed on blots.