Figure 6.

Knockdown of E-cadherin significantly reduces lung metastases following intravenous injection of 4T1 cells into BALB/c mice. (A) BLI imaging at 1, 3, or 8 days post-injection of 1*10⁵ 4T1-luciferase shCon or shEcad cells into the tail vein of BALB/c mice. (B) Quantification of total flux in the lungs of mice injected with 4T1-luciferase shCon or shEcad cells. Histograms indicate a major reduction in the development of lung metastases in the E-cadherin knockded-down cells. Statistical significance of the differences between the total flux in the lungs of the mice injected with the knocked-down and control cells was determined using two-way ANOVA, based on three independent experiments, n = 11 in each group (**p = 0.001).