Table 1.
Synergism between Cry11Aa and Cyt1Aa-hybrid proteins against 3th instar of Aedes aegypti larvae.
| Toxin | S(toxin) OBSa = (R1 + R2 + R3)/n | S(mixture) EXPb = S(Cyt1Aa)OBS × S(Cry11Aa)OBS | Expected mortalityc = (1 − S(mixture)EXP) × 100% | Observed mortality Cyt1Aa + Cry11Aad |
|---|---|---|---|---|
| Cyt1Aa | 1 | 0.76 | 24% | 97% ± 0.6 |
| CytL3-Loop4 | 1 | 0.76 | 24% | 70% ± 0 |
| CytL3-Loop5 | 1 | 0.76 | 24% | 80% ± 1.7 |
| CytL3-Loop6 | 1 | 0.76 | 24% | 67% ± 0.6 |
| CytL3-Loop7 | 1 | 0.76 | 24% | 87% ± 1.2 |
| CytL3-loop8 | 1 | 0.76 | 24% | 80% ± 1.0 |
| CytL3-Loop9 | 1 | 0.76 | 24% | 80% ± 1.0 |
| CytL3-Loop10 | 1 | 0.76 | 24% | 83% ± 2.1 |
| Cry11Aa | 0.76 |
aObserved survival of individual toxin S(toxin)OBS corresponds to the observed proportion of larvae that survived to the exposure to Cyt1Aa or Cyt1Aa-hybrid mutant toxins. Observed mortality was 20% with Cry11Aa at 108 ng per ml and 0% with Cyt1Aa or mutant toxins at 30 ng per ml. n = 30 larvae for each toxin tested.
bTheoretical proportion of larvae that survive to the toxin-mixture, S(Cyt1Aa, Cry11Aa)EXP = S(Cyt1Aa)OBS × S(Cry11Aa)OBS corresponds to the proportion of larvae expected to survive to the exposure of a mixture of toxins.
cTheoretical expected mortality was calculated with (1−S(Cyt1Aa, Cry11Aa)EXP) × 100%.
dExperimentally observed mortality with the mixture of toxins using Cry11Aa at 108 ng per ml plus each Cyt1Aa-hybrid toxin at 30 ng per ml.
These assays were performed three times. Fisher’s exact test showed values of P < 0.001 for each comparison.