Fig. 3.

The genomic locus encoding the VZV latency-associated transcript (VLT). a Schematic diagram showing the location and structure of the five VLT exons (blue blocks) and introns (blue lines) within the genomic region 101,000–106,000 (coordinates refer to VZV reference strain Dumas; NC_001348.1) (Supplementary Table 3). b The VLT mRNA sequence including the 5’ untranslated region; start and stop codons are highlighted in red italic, while the cleavage factor I (CFI)-binding motifs are highlighted in green italic and the canonical polyadenylation signal site (AATAAA) is underlined. Location and boundaries of VLT exons are indicated by vertical black lines. c The fully translated VLT protein (pVLT), with the sequence of peptide (red) used to produce rabbit polyclonal anti-pVLT antibody