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. 2018 Mar 21;9:1167. doi: 10.1038/s41467-018-03569-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Selective repression of VZV ORF61 gene expression by VLT in co-transfected cells. ARPE-19 cells were transfected with plasmids encoding FLAG-tagged VLT (VLT_ATG), mutated VLT in which the ATG start codon was replaced by ATA sequence (VLT_ATA) or empty control plasmid (empty) in combination with plasmids encoding ORF61, ORF62 and ORF63. a Analysis of VZV ORF61, ORF62 and ORF63 and human β-actin transcript by RT-qPCR. Data represent mean (±SEM) fold-change in gene expression using the empty vector to calibrate ORF61, ORF62 and ORF63 or using VLT_ATA to calibrate VLT_ATG. Data are from two independent experiments performed in duplicate. nd not detected. ***p < 0.001; one-way ANOVA with Bonferroni’s correction for multiple comparisons. b Western blot analysis using antibodies directed to proteins encoded by VZV ORF61 (IE61), ORF62 (IE62), ORF63 (IE63), VLT (pVLT) and FLAG-tagged pVLT and α-tubulin. None, untransfected ARPE-19 cells. Images are representative of four independent experiments