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. 2018 Mar 21;8:4975. doi: 10.1038/s41598-018-23176-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockout strategy and genotyping of mutant mice. (A) Schematic strategies for the generation of KO mice using CRISPR/Cas9. 44 bp of Tex37 (a), 11 bp and 20 bp of Ccdc73 (b) and 17 bp of Prss55 (c) were deleted from Exon 2. While, 18 bp and 32 bp of Lyzl1 (d) and 101 bp of Nxt2 (e) were deleted from exon 3. (B) Genotype of each KO mouse was confirmed with PCR. (C) Representative Sanger sequence image for the verification of each KO mouse. Red arrow heads above the chromatograms and dashes in aligned cds sequences show the deletions.