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. 2018 Mar 21;8:4975. doi: 10.1038/s41598-018-23176-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fertility and spermatogenesis of the KO mice. (A) Representative images of testes from 70-dpp-old WT and KO mice. Scale bars, 2 mm. (B) Testis/body weight ratio of 70-dpp-old WT and KO mice. Error bars represent SD. n, the number of animals. NS, no significant difference, Student’s t-test was performed between WT and each KO mouse group for testis/body weight ratio. (C) Sperm count of 70-dpp-old WT and KO mice. Error bars represent SD. n, the number of animals. NS, no significant difference, Student’s t-test was performed between WT and each KO mouse group for sperm count. (D) H&E staining of testes and epididymides caput and cauda from 70-dpp-old WT and KO mice. Scale bars, 100μm. The data shown is representative images from at least three mice.