FIG. 2.

IL2, IFNγ, and TNFα mRNA from virus-specific CD8⁺ T cells following autologous CD4⁺CD25⁺ Treg cell coculture. Virus-nonspecific and virus-specific CD8⁺ T cells from FIV⁺ cats were sorted as described in Figure 1. Virus-nonspecific CD8⁺ T cells (black bars, CFSE^hi) from FIV⁺ cats were cultured with autologous Treg cells (striped bars, CFSE^hi+Treg) and virus-specific CD8⁺ T cells from FIV⁺ cats (white bars, CFSE^low) were sorted with autologous Treg cells (gray bars, CFSE^low+Treg). Cells were isolated and cultured at 1 week (left column), 4 weeks (middle column), and 8 weeks (right column) postinfection. (A) Following Treg cell coculture, IL2 mRNA is decreased in virus-specific CD8⁺ T cells at 4 and 8 weeks postinfection. (B) Following Treg cell coculture, IFNγ mRNA is decreased in virus-specific CD8⁺ T cells at 4 and 8 weeks postinfection. (C) TNFα is decreased in virus-specific CD8⁺ T cells at 4 weeks postinfection following Treg cell coculture (n = 3–7 cats for each time point). Bars represent the mean + SEM. Data were analyzed using an unpaired t-test with significance set at p < .05, asterisks. SEM, standard error of the mean.