Figure 3.

γδ chain usage and memory phenotype in cultured γδ T cells. Phenotype of the cultured γδ T cells. The phenotype of the γδ T cells with regard to the expression of memory markers and specific γ and δ chains was assessed by flow cytometry. In (a), the percentage of γδ T cells positive for Vγ9 (left panel), Vδ2 (middle panel), and Vδ1 (right panel) is shown. The fold expansion of Vγ9⁺ T cells and Vδ1⁺ T cells is displayed in (b) and (c), respectively. In (b), the grey box in the left graph contains the UCB γδ T cells cultured with 5 μM zoledronate and 50, 100, or 200 IU IL-2/ml medium, and in the right graph, indicated by the arrow, the data inside the box is displayed separately for each cultured UB unit (each unit is indicated by a differently colored line). The memory phenotype of the γδ T cells before and after culture, using a definition based on the expression of CD45RO and CCR7, is displayed in (d). Naïve T cells were defined as CD45RO⁻ CCR7⁺, central memory as CD45RO⁺ CCR7⁺, effector memory as CD45RO⁺ CCR7⁻, and terminally differentiated cells were defined as CD45RO⁻ CCR7⁻. The memory phenotype, defined by the expression of CD45RO and CD27, is displayed in (e). Naïve T cells were defined as CD45RO⁻ CD27⁺, central memory T cells as CD45RO⁺ CD27⁺, effector memory T cells as CD45RO⁺ CD27⁻, and terminally differentiated T cells as CD45RO⁻ CD27⁻. IL-2: interleukin 2; TCM: central memory T cells; TCR: T cell receptor; TEM: effector memory T cells; ZOL: zoledronate.