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Recommended melting conditions on three qPCR platforms.
| Platforms | Recommended instrument setting | Measured ramp rate (°C/s) | Total time (min) |
|---|---|---|---|
| LightCycler 96 | 5 readings/°C | 0.05 | 30 |
| LightCycler 480 | 5 acquisitions/°C | 0.03 | 45 |
| Bio-Rad CFX-96 | Step (0.3°C)/hold (5 s) | 0.01 | 60 |
