Fig. 3.

Temperature, pH and thermostability dependence profiles. The effect of temperature (a) and pH (b) on the Chit42 chitinase activity was evaluated on colloidal chitin at pH 6 and 35 °C, respectively. c The chitinase was incubated for the indicated temperatures during the referred time periods (in min) prior to the addition of the substrate. Remaining activity was determined at 35 °C as described in the “Methods” section. Results represent the mean of three independent values. Standard errors are indicated