Table 3.

Sample specifications

			Water temperature on 5 days before sampling day (°C)
Sample number	Sampling date	Developmental stage	Mean	Maximum	Minimum	Median	Interval	Mean water temperature on sampling day (°C)	Sequencing library	Individual dissimilarity	Raw read pairs	QF read pairs	% retained	% total
2	15-May-15	8-cell stage	15.30	16.56	13.94	15.20	2.62	16.06	1	0.865	23,797,396	23,679,970	99.5	5.3
50	1-Jun-15	1 cell stage	15.54	16.38	14.48	15.63	1.90	16.49	1	0.805	47,705,400	47,500,770	99.6	10.7
28	22-May-15	4-cell stage	15.58	16.28	14.76	15.64	1.52	14.84	2	0.915	20,373,120	19,616,406	96.3	4.4
30	22-May-15	32-cell stage	15.58	16.28	14.76	15.64	1.52	14.84	1	0.76	26,604,709	26,292,764	98.8	5.9
70	3-Jun-15	16–32 cell stage	16.19	17.28	15.26	16.10	2.02	17.55	2	1.106	40,054,067	39,820,682	99.4	8.9
80	8-Jun-15	16–32 cell stage	17.00	18.68	15.72	16.90	2.96	19.01	2	0.9	50,809,855	50,559,355	99.5	11.4
132	26-Jun-15	4–8 cell stage	18.25	19.22	17.50	18.24	1.72	18.80	1	0.83	16,474,441	15,903,806	96.5	3.6
135	26-Jun-15	1-cell stage	18.25	19.22	17.50	18.24	1.72	18.80	1	1.165	31,063,597	30,915,991	99.5	6.9
98	5-Jun-15	1–2 cell stage	19.01	21.46	17.14	18.96	4.32	20.70	2	0.785	49,901,005	49,640,357	99.5	11.2
123	22-Jun-15	16-cell stage	19.03	19.74	18.22	19.08	1.52	18.35	2	1.015	21,550,669	21,364,189	99.1	4.8
127	22-Jun-15	2-cell stage	19.03	19.74	18.22	19.08	1.52	18.35	2	1.095	20,752,958	20,628,391	99.4	4.6
147	3-Jul-15	1-cell stage	20.66	23.06	19.42	20.38	3.64	22.54	1	1.12	20,273,141	20,149,106	99.4	4.5
148	3-Jul-15	16-cell stage	20.66	23.06	19.42	20.38	3.64	22.54	1	1.105	40,050,217	39,840,710	99.5	9.0
167	10-Jul-15	4-cell stage	23.95	24.60	23.30	23.98	1.30	23.16	2	0.76	24,849,517	24,685,544	99.3	5.5
141	3-Jul-15	prim stage	20.66	23.06	19.42	20.38	3.64	22.54	1	NA	14,498,531	14,346,106	98.9	3.2