Fig. 9.

Reverse Transcriptase–Quantitative PCR analysis of selected genes during on–vine ripening in ‘Sanuki Gold’ kiwifruit. Genes for analysis were selected based on (i) DEGs that were up–regulated by both propylene and low temperature (a, b, c, d); (ii) DEGs that were exclusively up–regulated by low temperature (e, f, g, h, i) and (iii) DEGs that were exclusively up–regulated by propylene (j, h, l, m, n, o). AdACTIN (EF063572) was used as the housekeeping gene and the expression of fruit at harvest (D0) was calibrated as 1. Values are means of three independent biological replicates. Error bars represent SE. Values are means of three independent biological replicates. Error bars represent SE. Different letters indicate significant differences at p < 0.05