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. 2018 Mar 2;9:408. doi: 10.3389/fimmu.2018.00408

Figure 1.

Figure 1

25D and 24,25D increases following CN of germ-free (GF) mice. (A) Vitamin D, (B) 25D, and (C) 24,25D at 2 and 4 weeks of +D supplementation. Values are ± SEM of n = 10 mice per group, five males and five females. Student’s t-test was used to test significance. (D) Experimental design of conventionalization (CN) and vehicle or +D supplementation. (E) Vitamin D, (F) 25D, and (G) 24,25D levels from plasma of GF before and after 2-week CN. Values are the mean ± SEM of n = 9 mice per group, four males and five females, and two independent experiments. Two-way ANOVA with Bonferroni post hoc tests was used to test significance (*P < 0.05, **P < 0.01, and ***P < 0.001).