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. 2018 Mar 6;9:321. doi: 10.3389/fmicb.2018.00321

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Copyright © 2018 Carvalho, Kloosterman, Manzoor, Caldas, Vinga, Martinussen, Saraiva, Kuipers and Neves.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Capsule promoter expression in D39 carA mutant exposed to different amounts of uracil. Transcription of Pcps was estimated by measuring β-galactosidase activities in exponentially growing carA mutant and wild-type strains, carrying the pPP2 integrative lacZ reporter plasmid, in Glc-CDM with 0 (0U), 5 (5U), and 10 (10 U) mg L⁻¹ uracil. β-galactosidase activities are expressed as Miller units. The values represent the means of the Pcps expression ratios ± SD obtained from two biological independent samples assayed in duplicate. ^**p-value = 0.0025.