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. 2018 Mar 6;9:321. doi: 10.3389/fmicb.2018.00321

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Copyright © 2018 Carvalho, Kloosterman, Manzoor, Caldas, Vinga, Martinussen, Saraiva, Kuipers and Neves.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Growth profiles of strains D39 wild-type and D39SM in co-cultivation. D39 and D39SM strains were inoculated at a cell ratio of 1:1 (□), 1:4 (▴), and 4:1 (•) in (A) CDM or (B) CDM without uracil, containing 1% Glc, and grown at 37°C, without pH control (initial pH 6.5), in static rubber-stoppered bottles. The growth rates of the co-cultures are presented in the graphs. The values are averages from three independent cultures ± SD. For comparison the growth curves and growth rates of monocultures of D39 (■) and D39SM (♦) are also shown. +U, with uracil; -U, without uracil.