Figure 4.

βcatenin and PAX6 transcriptional regulation of Ccnd1 regulative regions identified by SP8 ChipSeq. (A) represents the schema of the 5′ portion of the exon 1 fragment. The transcriptional start sites, TSS1 and TSS2, the first codon ATG, and the conserved βcatenin binding site (Klein and Assoian, 2008), and the SP8 summit (SP8S) positions are indicated. The 5′UTR region is depicted in black; the ORF of exon 1 is indicated in white. Panel (B) shows the results of the luciferase assay obtained from one representative experiment. P19 cells were transfected with the Ccnd1 exon fragments identified by SP8 ChipSeq alone or in combination with βcatenin, SP8, or βcatenin together with SP8. Statistical significance, ANOVA: ^**p < 0.01, ^***p < 0.001.