Table 1.

Proteotypic peptides for SRM assay

Protein	Amino acid	Target peptide sequences	Proteoform quantified	SRM transitions
GDF8	66–74	LETAPNISK	propeptide	486.8/558.3(y5);486.8/629.4(y6);486.8/730.4(y7)
	210–217	TVLQNWLK	propeptide	501.4/560.1(y4);501.4/688.2(y5)501.4/801.3(y6)
	218–228	QPESNLGIEIK	propeptide	614.3/260.2(y2);614.3/559.3(y5);614.3/672.4(y6)
	267–280	DFGLDC[CAM]DEHSTESR	mature	556.6/579.0(y5);556.6/716.2(y6);556.6/674.7(+3y11+2)
GDF11	90–96	EAPNISR	propeptide	393.7/293.7(y5+2);393.7/489.3(y4);393.7/586.3(y5)
	232–241	SGHWQSIDFK	propeptide	602.8/911.4(b8);602.8/737.4(y6);602.8/923.5(y7)
	253–265	YPHTHLVQQANPR	mature	521.0/457.02(+3y4);521.0/585.1(+3y5);521.0/713.1(+3y6)
	299–312	NLGLDC[CAM]DEHSSESR	mature	540.4/565.0(y5);540.4/611.1(y10+2);540.4/696.1(y12+2)
Follistatin	230–238	SIGLAYEGK	isoforms 1 & 2	469.3/496.0(y4);469.3/567.1(y5);469.3/737.2(y7)
	312–331	HSGSC[CAM]NSISEDTEEEEEDEDQ	FST303	809.5/843.3(b8);809.5/7262.0(y2);809.5/764.1(y6)
WFIKKN1	509–521	DGVAVLDAGSYVR	mature	661.3/767.1(y7);661.3/880.0(y8);661.3/1050.0(y10)
WFIKKN2	32–39	SLALPPIR	mature	433.9/482.1(y4);433.9/595.2(y5);433.9/666.2(y6)
	298–306	ADFPLSVVR	mature	502.3/573.1(y5);502.3/670.2 (y6);502.3/409.1(y7+2)
Oxytocin	20–28	C[CAM]YIQNC[CAM]PLG	mature	562.8/839.1(b6);562.8/286.1(y3);562.8/446.0(y4)
	20–29	C[CAM]YIQNC[CAM]PLGG	oxytocin-G	591.3/839.2(b6);591.3/343.1(y4);591.3/503.1(y5)
	20–30	C[CAM]YIQNC[CAM]PLGGK	oxytocin-GK	655.4/745.1(y7);655.4/873.2(y8);655.4/986.3(y9)
Eotaxin	41–45	IPLQR	mature	313.8/257.1(y4+2);313.8/416.1(y3);313.8/513.1(y4)
	87–91	YLDQK	mature	333.7/275.1(y2);333.7/390.0(y3);333.7/503.1(y4)