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Transgenic GAD67-GFP mice display immunofluorescence consistent with a Type III cell population. Confocal z-stack images of (A) fungiform and (B) circumvallate taste buds from a GAD67-GFP transgenic mouse showing GAD67-GFP fluorescence in green and PLCβ2 immunoreactivity in magenta. Scale bars = 20 µm. GAD67-GFP-positive taste cells do not coincide with the Type II cell marker PLCβ2 in any taste field, nor do they share morphology with Type I cells.
