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. 2018 Mar 2;15(4):3705–3714. doi: 10.3892/etm.2018.5920

Figure 8.

Figure 8.

Titer measurement of lentivirus expressing hsa-miR-34a. (A) Bright field image of group with 10 µl lentivirus. (B) Green fluorescent image of group with 10 µl lentivirus. (C) Bright field image of group with 1 µl lentivirus. (D) Green fluorescent image of group with 1 µl lentivirus. (E) Bright field image of group with 0.1 µl lentivirus. (F) Green fluorescent image of group with 0.1 µl lentivirus. Magnification, ×100. Virus titer was calculated using the following formula: (Tu/ml)=(fluorescent cell number × virus dilution fold)/0.1. Virus titer of hsa-miR-34a was 4×108 Tu/ml. miR-34a, microRNA-34a.