Fig. 2. 19F NMR spectra of 19F- labelled wild-type and W24F mutant βPGM. Diamonds (♦) are 5FW216 resonances and circles (•) are free MgF3– resonances (–155 ppm). Resonances at –119 are from free F–. Assignment of MgF3– and AlF4– resonances were previously determined by chemical shift.21,33 Assignment of MgF3– and AlF4– resonances were previously determined by chemical shift.21,33 (A) 5FWβPGM; (B) W24F, 5FW216 5FWβPGM; (C) W24F, 5FW216 5FWβPGM–MgF3–G6P TSA complex.
