Table 1. Identification by mass spectroscopy of larval and adult Haemonchus contortus proteins eluted from LGALS-11 and LGALS-14 columns.
| Accession code | Gene description | CV | PSMs | UP | Mascot score | Groups | Log-odds | NSAF | MW (kDa) | Signal peptide | Number of N-and (blue)/ or O-glycosylation sites (red)a | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | |||||||||||
| Metabolic process | ||||||||||||||
| W6NE18 | Peptidase S28 GN=HCOI_01497800 | 45.21 | 634 | 20 | 7,058 | No | Yes | Yes | Yes | 145.70 | 0.071 | 67.1 | No | 1/5 |
| W6NFG0 | Alpha beta hydrolase fold-1 GN=HCOI_00457700 | 58.65 | 504 | 16 | 6,681 | No | Yes | Yes | Yes | 128.68 | 0.129 | 36.6 | Yes | 1/0 |
| W6NFT9 | Peptidase S28 GN=HCOI_01562400 | 23.27 | 222 | 18 | 3,151 | No | Yes | No | Yes | 11.26 | 0.0062 | 128.6 | Yes | 7/35 |
| W6NJ96 | Carboxyl transferase GN=HCOI_00766200 | 23.39 | 123 | 10 | 1,146 | No | Yes | No | Yes | 16.01 | 0.0103 | 56.1 | No | 0/7 |
| W6NLA8 | Glutamate phenylalanine leucine valine dehydrogenase GN=HCOI_01838800 | 42.50 | 102 | 10 | 957 | No | Yes | No | Yes | 20.61 | 0.020 | 26.8 | No | 0/0 |
| W6NG90 | Peptidase S28 GN=HCOI_01624000 | 4.88 | 101 | 3 | 1,218 | No | Yes | No | Yes | 29.6 | 0.003 | 117.0 | Yes | 4/7 |
| W6NC58 | Aldo keto reductase GN=HCOI_00043700 | 39.64 | 83 | 10 | 789 | No | Yes | No | Yes | −0.18 | 0.009 | 38.0 | No | 3/6 |
| W6NAV8 | Aldo keto reductase GN=HCOI_00043500 | 27.22 | 46 | 6 | 477 | No | Yes | No | Yes | −0.18 | 0.0028 | 40.0 | No | 2/4 |
| W6NU27 | Carboxyl transferase GN=HCOI_00766300 | 25.83 | 67 | 6 | 909 | No | Yes | No | Yes | 4.35 | 0.0023 | 42.2 | No | 1/0 |
| W6NKM1 | Succinate dehydrogenase iron-sulfur subunit, GN=HCOI_01735500 | 32.61 | 64 | 8 | 521 | No | Yes | No | Yes | 34.83 | 0.0067 | 31.6 | No | 1/10 |
| W6NF70 | Deoxynucleoside kinase GN=HCOI_01673500 | 35.42 | 42 | 6 | 242 | No | No | No | Yes | −0.18 | 0.0012 | 34.8 | No | 0/0 |
| U6NNG6 | Ribosomal protein L7 L12 GN=HCOI_00340500 | 11.41 | 30 | 1 | 783 | No | Yes | No | Yes | −0.18 | 0.013 | 19.5 | No | 1/3 |
| W6NA79 | Zinc metallopeptidase M13 GN=HCOI_01030800 | 37.90 | 28 | 3 | 330 | No | No | No | Yes | 4.35 | 0.0067 | 14.6 | No | 0/2 |
| W6ND82 | von Willebrand factor domain containing protein GN=HCOI_01354500 | 13.82 | 26 | 3 | 371 | No | No | No | Yes | 16.01 | 0.0077 | 27.5 | Yes | 2/0 |
| W6NMI7 | Proteinase inhibitor I33 GN=HCOI_02015200 | 18.58 | 22 | 5 | 162 | No | No | No | Yes | −0.18 | 0.0020 | 25.1 | Yes | 0/7 |
| W6NKG5 | Ribosomal protein L15 GN=HCOI_01717500 | 5.88 | 4 | 1 | 26 | No | No | No | Yes | 5.67 | 0.0002 | 24.3 | No | 1/10 |
| W6NEW9 | Amidinotransferase GN=HCOI_01556200 | 23.35 | 24 | 4 | 109 | No | Yes | No | No | −0.18 | 0.0014 | 29.2 | No | 0/0 |
| W6NI22 | Adenylosuccinate lysase GN=HCOI_00436500 | 6.41 | 14 | 3 | 141 | No | Yes | No | No | 12.23 | 0.0005 | 76.9 | No | 1/4 |
| W6NF84 | Short-chain dehydrogenase reductase GN=HCOI_01467500 | 3.07 | 11 | 1 | 137 | No | Yes | No | Yes | 1.71 | 0.0003 | 27.5 | No | 0/0 |
| W6ND43 | Acetyltransferase component of pyruvate dehydrogenase complex GN=HCOI_00576100 | 1.22 | 8 | 1 | 51 | No | Yes | No | No | 1.68 | 0.0001 | 78.3 | No | 1/40 |
| Regulation of biological processes | ||||||||||||||
| W6NC73 | ATPase GN=HCOI_02138200 | 22.64 | 23 | 2 | 418 | No | Yes | No | Yes | −0.18 | 0.004 | 17.8 | No | 1/0 |
| W6NJ12 | Filament domain containing protein GN=HCOI_02013700 | 14.09 | 20 | 3 | 291 | No | Yes | No | Yes | −0.18 | 0.0028 | 34.2 | No | 0/4 |
| W6NM02 | Fumarate lyase GN=HCOI_01914600 | 4.73 | 8 | 1 | 62 | No | Yes | No | No | 4.61 | 0.0003 | 29.7 | No | 1/12 |
| W6NGK5 | CRE-DHS-15 protein GN=HCOI_00341400 | 29.31 | 6 | 1 | 210 | No | No | No | Yes | −0.18 | 0.0031 | 6.1 | No | 0/1 |
| W6NI80 | Acyl-CoA-binding protein GN=HCOI_01539100 | 20.69 | 6 | 1 | 205 | No | Yes | No | Yes | 6.69 | 0.0018 | 9.5 | No | 0/1 |
| W6NCC1 | NIPSNAP GN=HCOI_01963300 | 7.63 | 6 | 1 | 75 | No | Yes | No | No | −0.18 | 0.0013 | 26.5 | No | 0/6 |
| W6NWY9 | Porin domain containing protein GN=HCOI_01573900 | 43.97 | 82 | 9 | 1,149 | No | Yes | No | Yes | 4.35 | 0.011 | 30.2 | No | 3/0 |
| W6NAW4 | FG-GAP and Integrin alpha-2/Integrin alpha chain GN=HCOI_01903100 | 5.90 | 33 | 5 | 340 | No | Yes | No | Yes | −0.18 | 0.0028 | 133.9 | Yes | 5/18 |
| W6NAL4 | Heat shock protein 70 GN=HCOI_00589700 | 7.24 | 9 | 1 | 82 | No | Yes | No | No | 7.23 | 0.0002 | 72.4 | No | 3/7 |
| Transport | ||||||||||||||
| W6NVQ1 | Lipid transport protein and Vitellinogen and von Willebrand factor domain GN=HCOI_01683400 | 23.18 | 209 | 24 | 1,465 | No | Yes | No | Yes | 9.62 | 0.0039 | 156.0 | No | 0/11 |
| W6N9I2 | Lipid transport protein GN=HCOI_00072100 | 35.97 | 139 | 13 | 1,313 | No | Yes | No | Yes | 51.51 | 0.0136 | 56.8 | Yes | 0/2 |
| W6NQZ5 | Mitochondrial substrate solute carrier GN=HCOI_01092000 | 23.10 | 53 | 8 | 334 | No | No | No | Yes | 6.2 | 0.0033 | 36.3 | No | 0/3 |
| Cytoskeleton | ||||||||||||||
| W6NAH7 | Nematode cuticle collagen and Collagen triple helix repeat GN=HCOI_00810700 | 5.60 | 85 | 2 | 1,399 | No | Yes | No | Yes | 4.35 | 0.0117 | 32.8 | No | 0/15 |
| W6NHH0 | Annexin GN=HCOI_01003500 | 14.01 | 22 | 3 | 135 | No | Yes | No | No | −0.18 | 0.0008 | 29.4 | No | 0/1 |
| W6NE41 | Myosin tail GN=HCOI_01216000 | 3.99 | 17 | 3 | 59 | No | Yes | No | No | 13.42 | 0.0008 | 54.7 | No | 0/15 |
| W6NF56 | Myosin tail GN=HCOI_01461300 | 34.48 | 51 | 8 | 675 | No | Yes | No | Yes | 6.2 | 0.0040 | 36.5 | No | 0/14 |
| Immunomodulatory | ||||||||||||||
| W6NGA7 | SCP extracellular domain GN=HCOI_01577700 | 14.95 | 4 | 2 | 59 | No | Yes | No | Yes | −0.18 | 0.0011 | 22.2 | No | 1/1 |
| Unknown | ||||||||||||||
| W6NAN9 | CBN-MLC-3 protein GN=HCOI_01274700 | 49.67 | 63 | 7 | 465 | No | Yes | No | Yes | 2.32 | 0.0086 | 17.0 | No | 0/2 |
| W6NX42 | Uncharacterized protein GN=HCOI_01051700 | 25.56 | 38 | 3 | 607 | No | No | No | Yes | 19.09 | 0.0066 | 25.5 | No | 4/40 |
| W6NFI4 | Protein C15F1.2 GN=HCOI_01126300 | 34.87 | 20 | 4 | 97 | No | Yes | No | Yes | −0.18 | 0.0022 | 27.1 | Yes | 0/13 |
| W6NB42 | Protein C23H5.8, isoform-c GN=HCOI_00648100 | 14.43 | 16 | 2 | 170 | No | Yes | No | Yes | 10.80 | 0.0025 | 22.5 | Yes | 1/1 |
| W6NPK3 | Uncharacterized protein GN=HCOI_00260500 | 5.20 | 10 | 1 | 45 | No | No | No | Yes | −0.18 | 0.0001 | 22.6 | Yes | 3/11 |
| W6NUX4 | Uncharacterized protein GN=HCOI_01608400 | 4.04 | 12 | 1 | 43 | No | Yes | No | Yes | 4.04 | 0.0002 | 21.8 | Yes | 0/6 |
Notes.
- GN
- Gene name
- CV
- Coverage
- PSMs
- Peptide spectrum matches
- UP
- Unique peptides
- Log-odds 0
- 50% chance
- Positive values
- More than 50%
- Negative values
- Less than 50%
Groups: 1, LGALS-11 bound protein from L4 larval stage of H. contortus; 2, LGALS-11 bound protein from adult stage of H. contortus; 3, LGALS-14 bound protein from L4 larval stage of H. contortus; 4, LGALS-14 bound protein from adult stage of H. contortus.
Predicated glycosylation sites as determined in materials and methods.
NSAF, Normalized Spectral Abundance Factor.