Figure 3. B. dubia survival is proportional to the F. tularensis LVS infection dose.
Groups of juvenile OS cockroaches (n=8) were injected with either PBS (no bacteria) or serial dilutions of F. tularensis LVS (1.7 × 106 to 103 CFU), incubated at 37°C, and monitored for survival over the course of 7 days. LVS was grown on CHOCII agar for 48 hours, harvested, and diluted in PBS. Statistical differences between groups were calculated by log-rank (Mantel-Cox) analysis. Asterisks indicate significant difference from the no bacteria control group (***=p<0.001).