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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: J Immunol. 2018 Jan 8;200(4):1335–1346. doi: 10.4049/jimmunol.1601870

Figure 1.

Figure 1

Phenotype of aged dcCasp8−/− mice. A) Deletion of exon 3 of caspase-8 was assessed in CD11c+ splenocytes from Casp8fl/fl (control), Casp8fl/fl Cd11cCre (dcCasp8−/−), and Casp8−/− Ripk3−/− mice by PCR. B) The expression of CD86 in splenic cDCs from control and dcCasp8−/− mice was determined at 3, 6, 10 and 14+ months (left), and the percentage of cDCs expressing high levels of CD86 is depicted (right). C) The expression of CD62L and CD44 in splenic and blood T cells from control and dcCasp8−/− mice was determined at 3, 6, 10 and 14+ months (left), and the percentage of T cells that were naive (CD62LhiCD44lo) or activated (CD62LloCD44hi) is depicted (right). D) Spleens and livers from 10-month old control and dcCasp8−/− mice were sectioned and stained with hematoxylin & eosin (H&E). The percentage of white pulp area per spleen section in control vs. dcCasp8−/− mice is depicted (right). E) The expression of Foxp3 in splenic CD4+ T cells from 10-month-old control and dcCasp8−/− mice was determined by percentage (left) and absolute number per spleen (right). Data for A, B, D and E are representative of three independent experiments for each time point, with n = 4. Data for C are representative of 16 mice. Error bars represent S.E.M.