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. Author manuscript; available in PMC: 2019 Feb 15.
Published in final edited form as: J Immunol. 2018 Jan 8;200(4):1335–1346. doi: 10.4049/jimmunol.1601870

Figure 7.

Figure 7

Enhanced IRF3 activation in RIG-I stimulated caspase-8 deficient BMDCs and splenic cDCs. A) Control and Casp8fl/fl Cd11cCre BMDCs were transfected with short-length poly(I:C) for the indicated lengths of time, then total cellular lysates were immunoblotted for P-IRF3. The membrane was then stripped and reblotted for total IRF3 and LaminB as a loading control. B) CD11c+ cells were isolated from the spleen and transfected with short-length poly(I:C). After 4 hours, the expression of P-IRF3 was assessed in CD11b+ and CD11b+CD86+ cDCs. Data for A are representative of two independent experiments. Data for B are representative of two independent experiments, with three replicates per genotype per experiment. Error bars represent S.D.