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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Int J Mass Spectrom. 2017 Sep 1;427:4–10. doi: 10.1016/j.ijms.2017.08.016

Figure 2.

Figure 2

Evaluation of the sensitivity of 30-μm i.d. column LC and LTQ-Orbitrap MS with various sample loading amounts from 0.5 ng to 50 ng tryptic digest of S. oneidensis. (a–b) Peptide and protein identification results with (a) MS/MS only and (b) combined MS/MS with match between runs (MBR). For each condition, at least two replicates were analyzed and averaged to generate the results. LC conditions: 30-μm i.d. column operated at 60 nL/min and a 150-min gradient from 5% to 28% Buffer B