Figure 4.

Residues 40–49 Form an NTE-Specific Kinase Autoinhibitory Module

(A) In vitro kinase assay of MYC-GFP-Mps1 constructs. Coomassie blue staining indicated equal loading. Quantification of Mps1 autophosphorylation is shown; data are means ± SEM; n = 3. ^∗p < 0.05 and ^∗∗p < 0.01.

(B) Immunoblotting with the indicated antibodies of the various MYC-Mps1 mutant proteins.

(C) MYC immunoprecipitates from cells expressing the indicated MYC-Mps1 constructs were resolved by SDS-PAGE and immunoblotted as shown.

(D) Cells were treated as in (C), enriched in mitosis with nocodazole, and immunoblotted as indicated.

See also Figures S4 and S5.