Fig. 2.

Effect of PHF5A knockdown on cell proliferation and colony formation in H1299 cells. a Phf5a mRNA levels from four common LAC cell lines were assessed by qRT-PCR. b-d Lentivirus-mediated shPHF5A knockdown in H1299 cells was performed via lentiviral infection (b), and qRT-PCR (c) and Western blot (d) were used to assess silencing efficacy. e Cellomics assay images and fluorescence quantification over five days in shCtrl and shPHF5A-transfected H1299 cells. f Colony formation assay was used to evaluate H1299 cell growth after PHF5A knockdown. ^**P < 0.01; ^**compared with the shCtrl group (e)