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. 2018 Mar 22;37:65. doi: 10.1186/s13046-018-0736-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Effect of PHF5A knockdown on cell cycle progression and apoptosis in H1299 cells. a Cell cycle was assessed in H1299 cells by flow cytometry five days after transfection with the indicated shRNAs. Representative flow-cytograms are shown, as well as diagrams quantifying cell fractions in the G₀/G₁, S, and G₂/M phases. b Apoptosis was evaluated by flow cytometry in PHF5A knockdown and control H1299 cells. Representative flow-cytograms are shown, and apoptotic rates were derived as the percentages of Annexin V-APC positive cells. ^**P < 0.01