Fig. 4.

Effect of PHF5A knockdown on cell proliferation, cell cycle, and apoptosis in H1975 cells. a-c shRNA against Phf5a was conducted by lentivirus infection, and Phf5a silencing efficacy in H1975 cells was determined at both mRNA (qRT-PCR) and protein (Western blot) levels. d During five days of continuous cell counting by fluorescence microscopy, the quantity of shPHF5A-transfected cells decreased gradually compared with control values. e The clonogenic ability of H1975 cells was declined after PHF5A knockdown as evidenced by colony formation assay. f Cell cycle analysis using FACS in shCtrl and shPHF5A-transfected H1975 cell lines. g Apoptosis was analyzed in PHF5A knockdown and control H1975 cells by FACS after Annexin V staining. ^*P < 0.05; ^**P < 0.01