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. 2018 Mar 22;37:65. doi: 10.1186/s13046-018-0736-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — Microarray analysis of H1299 cells after PHF5A knockdown. a Hierarchical cluster analysis of shCtrl and shPHF5A-transfected H1299 cells. A total of 1112 genes were differentially expressed. Heat-map colors represent mean-centered fold change expression in log-scale. b Disease and function enrichment analysis based on gene numbers, expression levels, and significance probability suggested that among the retrieved categories, genes related to “Cancer” ranked first with the greatest changes. c Pathway enrichment analysis for related genes, based on significance probability, suggested that among the analyzed gene sets, genes related to the IGF-1 signaling pathway ranked second in showing the greatest changes in expression levels. d Fold changes of three putative IGF-1 pathway genes, including IGFBP3, PIK3CB, and AKT2, as well as cell cycle/apoptosis related genes, including DDIT3, Skp2, and P53, based on microarray analysis. e-f Validation of microarray data by qRT-PCR (e) and Western blot (f). IGFBP3, DDIT3, P53 were significantly up-regulated, while PIK3CB, AKT2, and Skp2 were significantly down-regulated upon PHF5A knockdown. g Knowledge-based interactive network for the selected targets in the IGF-1 pathway as well as cell cycle/apoptosis related molecules was constructed with the Reactome database. Red and green denote up-regulated and down-regulated genes, respectively. NMJ, neuromuscular junction (c). ^*P < 0.05; ^**P < 0.01, compared with the shCtrl group. A higher quality of Fig. 7 is available as Additional file 6