Table 2.
Body weight, adiposity-related parameters, serum parameters, and HOMA-IR index
| NF | HF45 | HF60 | |||||
|---|---|---|---|---|---|---|---|
| Body weight (g) | 477 (27) | 466 (23) | 472 (32) | ||||
| Fat mass (%) | 18.4 (3.3) | a | 20.5 (4.8) | a,b | 23.4 (4.5) | b* | |
| Adiposity index (%) | 9.32 (1.51) | a | 11.2 (2.5) | a,b | 13.5 (2.7) | b* | |
| Weight of adipose tissues (g) | |||||||
| eWAT | 14.0 (2.1) | a | 16.0 (4.5) | a,b | 18.1 (5.5) | b* | |
| iWAT | 11.9 (2.5) | a | 16.1 (4.6) | a,b* | 15.7 (5.0) | b* | |
| mWAT | 6.89 (1.87) | 7.05 (2.92) | 7.90 (2.18) | ||||
| rWAT | 11.9 (3.2) | a | 13.6 (4.2) | a,b | 17.6 (6.1) | b* | |
| Visceral fat content (g) | 32.1 (6.9) | a | 36.6 (10.9) | a,b | 43.6 (13.1) | b* | |
| Liver fat content (mg/ g liver) | 38.3 (6.2) | a | 57.9 (15.4) | b* | 56.2 (11.9) | b* | |
| Glucose (mg·dl−1) | Feeding | 93.3 (9.4) | a | 106 (5.5) | b* | 111 (13.3) | b* |
| Fasting | 89.6 (7.4) | a | 98.4 (8.4) | b* | 97.4 (7.1) | b* | |
| Insulin (μg·l−1) | Feeding | 2.21 (0.85) | 1.88 (0.89) | 1.68 (0.67) | |||
| Fasting | 0.32 (0.11) | a | 0.51 (0.28) | a,b | 0.80 (0.37) | b* | |
| HOMA-IR | 1.7 (0.7) | a | 2.8 (1.6) | a,b | 4.5 (2.1) | b* | |
Data correspond to male Wistar rats fed a control diet (NF), a moderate high-fat diet (45% kcal from fats, HF45 group), or a very high-fat diet (60% kcal from fats, HF60 group) from the age of 2 months until the age of 6 months. Food in HF groups was offered in isocaloric amounts to the control group
Abbreviations: eWAT epididymal white adipose tissue; iWAT inguinal WAT; mWAT mesenteric WAT; rWAT retroperitoneal WAT
Results represent mean (SD) (n = 10 in all groups). Statistics: *different vs control group (Student’s t test, p < 0.05). A one-way ANOVA was performed followed by an LSD post hoc test. Values not sharing a common letter (a, b) are significantly different (p < 0.05). No letters = no statistical difference