Fig. 4.

TP53TG1-induced cisplatin sensitivity of NSCLC cells was decreased following miR-18a upregulation. A549/DDP cells were transfected with pcDNA-TP53TG1 alone or together with miR-18a mimics, followed by qRT-PCR assay of miR-18a expression (a), MTT analysis of IC50 of cisplatin (c) and flow cytometry analysis of apoptotic rate (e). A549 cells were introduced with si-TP53TG1#1 alone or together with anti-miR-18a, followed by measurement of miR-18a expression by qRT-PCR (b), determination of IC50 of cisplatin by MTT (d), detection of apoptotic rate by flow cytometry (f). Each experiment is repeated three times. *P < 0.05 vs. corresponding control