Figure 6.

Fluorescence images of MCF7 cells cultured in monolayer electroporated using a pair with needles (A) 1 cm distant, (B) 2 cm distant with 4 + 4 pulses, and (C) 2 cm distant with 24 + 24 pulses. The panel shows the reconstructed microscope images captured in the area including the 2 needles. The red fluorescence indicates cells positive to propidium iodide (PI; cells electroporated); blue fluorescence indicates cells positive to calcein blue AM (CB; cells viable); stars indicate the position of needle in the cell culture.