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. 2018 Mar 22;19:39. doi: 10.1186/s13059-018-1413-5

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Comparison of different dCas9/gRNA systems for labeling the endogenous human MUC4 genomic locus. a A gRNA targeting the human MUC4 gene was transfected together with dCas9 (upper panel) or without dCas9 (lower panel). b Upper panel: histograms of dCas9/gRNA foci formation efficiency in different dCas9/gRNA labeling systems (measured as % of GFP-positive cells, N > =20) in the condition with dCas9 (black bars) and without dCas9 (gray bars). Lower panel: dot plots of signal-to-noise ratio in the condition with dCas9 (black dots) and without dCas9 (gray dots). Each dot represents average value of all foci in one cell