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Type 17 immunity is increased in STAT1−/− mice. WT or STAT1−/− mice were infected with influenza A/PR/8/34 or vehicle for 6 days prior to infection with MRSA (USA300) or vehicle for 24 hours. A, B) Type 17 cytokine gene expression by RT-PCR on lung RNA (n = 11–12). C – F) Type 17 cytokine producing cells by flow cytometry (n = 8). F = influenza infected, SA = MRSA infected, FS = influenza MRSA super-infected. *p < 0.05 vs. WT for each treatment
