Fig 6. Prostaglandin receptor (EP₄) expression and inhibition of AgNP-directed mast cell degranulation.

Bone marrow-derived mast cell (BMMCs) isolated from C57BL/6J mice were evaluated for degranulation by measuring ß-hexosaminidase release following 1 h exposure of AgNP at 25 μg/ml ± pre-treatment with EP₄ selective inhibitor (10 μM), prostaglandin (PGE₂, 10 μM), or Cox-1 and -2 inhibitors (indomethacin, 5 μM). Values are expressed as mean ± SEM (n = 3). * Indicates significant difference compared to non-treated control and ^# indicates significant difference compared to AgNP treatment alone (p ≤ 0.05).