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. 2018 Mar 22;13(3):e0194068. doi: 10.1371/journal.pone.0194068

Table 3. Locus data.

Locus Primers PCR Annealing Conditions Reference Locus Name Genbank Accession Number
Cytb F 5’ TGA CTT GAA RAA CCA YCG TTG 3’ 58°C for 40 sec. Rocha-Olivares et al. (1999) Cytochrome b mitochondrial JX886053-JX886194
R 5’ATA TCA TTC TGG CTT AAT GTG 3’
Ets F 5’ CGG CCA TGG GCA GTT CAG G 3’
R 5’ATA TGC TTA AAT TCA GCG GG 3’
S7 F 5’ AGCGCCAAAATAGTGAAGCC 3’ 60 and 58°C for 60 sec. Chow and Hazama (1998) and This paper S7 Ribosomal, intron 2 JX886479-JX886620
R 5’ GCCTTCAGGTCAGAGTTCAT 3'
C272 R 5’ CAT CTA CTG ACA CTT GTA TAC TA 3’ (internal with S7 – F)
Mdh F1 5’ CCT CTC TCA CTG CTG CTG AA 3’ 61°C for 30 sec. This paper malate dehydrogenase, coding JX886337-JX886478
R1 5’ TTC TTC TCG ATG CCG TTC TT 3’
RF R2 5’ TCC CCA GAA GAA GAG GTG TG 3’ (internal with Mdh – F1)
Mep F 5’ GCT GTA ATG GAA TGG GCA TCC 3’ 60°C for 60 sec. This paper malic enzyme, intron JX886621-JX886762
R 5’ AGC CTC TCC AGC TCC CTG G 3’
RF R2 5’ GGT TAA CTT TAT GGC ATT ATG AAG AA 3’ (internal with Mep – F) 60°C for 40 sec.
RF F2 5’ TTG GAA ACC ACA ATG CCT TC 3’
RF R3E 5’ CAC GGT AAA CAA TGA AGT AT CTG 3’ 58°C for 60 sec