Table 1. HBV genetic diversity in three infected individuals characterized by sequencing whole genome PCR products using three different methods.

Sample	HBV genotype	Direct Sanger sequencing	Molecular cloning	Direct MinION sequencing
B5584	D1/E	Max. sequence length: 3,209-nt	Max. sequence length: 3,209-nt	Max. sequence length: 3,201 nt
		D1/E recombinant sequence	Individual D1/E recombinant sequences	Individual D1/E recombinant sequences
		Single nucleotide ambiguities across whole genome	D1 and E parental sequences not detected	D1 and E parental sequences not detected
				Single nucleotide ambiguities across whole genome*
				1,229-nt deletion (pos. 2,471–489)—putative spliced variant
B6505	A2	Max. sequence length: 3,220-nt	Not done	Max. sequence length: 3,217-nt
		Mixed population of wild-type and single nucleotide mutant S sequences		Mixed population of wild-type and single nucleotide mutant S sequences
				Evaluation of mutation association patterns
				1,049-nt deletion (pos. 2,447–282)—putative spliced variant
				96-nt deletion (pos. 2,131–2,226)
B6260	D2	Overlapping sequence signals suggesting at least 2 sequences coexisting	Individual wild-type sequences (3,179-nt)	Max. sequence length: 3,015-nt
		Single nucleotide ambiguities across whole genome	Individual sequence (3,032-nt) with 123-nt (pos. 2,929–3,051) and 24-nt (pos. 501–524) deletions	Individual wild-type sequences
				Individual sequences with 123-nt (pos. 2,929–3,051) deletion
				Individual sequences with 123-nt (pos. 2,929–3,051) and 24-nt (pos. 501–524) deletions
				Single nucleotide ambiguities across whole genome*

* Single nucleotide sequence ambiguities were not investigated further.

Abbreviations: gt, genotype; nt, nucleotide; pos, position.