Table 2. LAST read mapping statistics for HBV sequencing runs on the MinION™ device.
| LASTa | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Total reads | Pass reads | Sanger sequence length (nt) | No of mapped reads | Mean Alignt read length (nt) | Max Alignt read length (nt) | Mean Alignt Identity (min-max) | Mean Miscall rate | Mean Insertion rate | Mean Deletion rate | Mean total error rate | No of reads covering ≥ 95% of the Sanger sequence | Mean total error rate (sequences covering ≥ 95% of the Sanger sequence) | |
| B5584c | 7,656 | 1,875 | 3,209 | 1,618 | 3,060 | 3,209 | 74.8% | 12.25% | 3.8% | 10.2% | 26.25% | 1,439 | 25.6% |
| (59.4–91.6) | (1.9–23.1) | (0.4–11.2) | (1.9–25.9) | (8.5–42.2) | (8.5–41.3) | ||||||||
| B6260c | 25,562 | 4,962 | 3,032 | 4,190 | 2,848 | 3,032 | 73.6% | 13.0% | 3.65% | 10.8% | 27.4% | 3,545 | 27.05% |
| (58.9–93.6) | (0.9–26.8) | (0.0–12.1) | (2.7–26.7) | (6.4–43.2) | (8.4–43.1) | ||||||||
a version 475
No: numberAlignment length values are rounded to the nearest whole number.