Table 3. Read mapping statistics for HBV sequencing runs after error correction using NanoCorrect.
NanoCorrect + LAST | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Pass reads | Sanger sequence length (nt) | No of corrected Pass reads | No of mapped reads | Mean Alignt read length (nt) | Max Alignt read length (nt) | No of reads covering ≥ 95% of the Sanger sequence | Mean Alignt Identity (min-max) (sequences covering ≥ 95% of Sanger) | Mean Miscall rate (sequences covering ≥ 95% of Sanger) | Mean insertion rate (sequences covering ≥ 95% of Sanger) | Mean Deletion rate (sequences covering ≥ 95% of Sanger) | Mean total error rate (sequences covering ≥ 95% of Sanger) | |
B5584 | 1,875 | 3,209 | 1,673 | 1,485 | 2,892 | 3,209 | 1,065 | 86.7% | 5.2% | 1.8% | 4.6% | 11.6% |
(72%)* | (60.2–99.0) | (0.0–15.7) | (0.0–7.9) | (1.0–14.0) | (1.1–32.3) | |||||||
B6260c | 4,962 | 3,032 | 3,975 | 3,509 | 2,555 | 3,032 | 1,993 | 88.2% | 5.6% | 2.1% | 4.5% | 12.3% |
(57%)* | (67.3–98.7) | (0.0–19.5) | (0.0–8.6) | (1.1–12.2) | (1.4–34.0) |
*Percentage calculated against the number of corrected and mapped reads
No: Number
Alignment length values are rounded to the nearest whole number.