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. 2018 Mar 22;13(3):e0194812. doi: 10.1371/journal.pone.0194812

Fig 1. Generation of targeted mutations in the CNP-encoding gene Nppc by ZFN.

Fig 1

(A) Schematic representation of the CNP protein and the target site within it (arrow). The empty (white) portion represents the signal peptide (23 a.a.), the filled (black) portion represents the NT-proCNP (50 a.a.), and the gray portion indicates the mature CNP with either 53 or 22 a.a. (B) Schematic representation of the target site in the rat Nppc (NC_005108.4) locus. (C) Schematic representation of the locations of PCR primers for genotyping.