Fig 2.

a) Effect of NT4 on migration of HUVEC on different coatings in a cell layer wound healing assay, measured as % of gap closure. HUVEC were plated on wells coated with collagen IV, fibronectin or on uncoated wells where a silicon spacer had been placed immediately before cell plating. Once cells had reached confluence, the silicon spacer was removed and cells were treated with NT4 peptide (10 μM) for 24 hours; b) Complete gap closure on fibronectin, untreated cells and C) impairment of HUVEC gap closure by NT4 on fibronectin.